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Fluidigm 시스템을 이용한 고추 유전자원의 주요병 유전형 평가

Evaluation of Genotypes for Major Diseases of Pepper Genetic Resources Using the Fluidigm System

금보라(Bora Geum) , 노나영(Na Young Ro) , 허온숙(On sook Hur) , 왕샤오한(Xiaohan Wang) , 최유미(Yu-Mi Choi) , 고호철(Hocheol Ko) , 이수경(Sukyeung Lee) 저

pp.432~449 (18pages)

Publisher : KOREAN JOURNAL OF BREEDING SCIENCE
Volume/Number : KOREAN JOURNAL OF BREEDING SCIENCE Vol.53 NO.4 / 2021
Publisher : The Korean Breeding Society
Publication : Journal
Format : PDF

Abstract

English
In this study, genotype analysis was conducted using the Fluidigm system with 13 SNP markers related to anthracnose, powderymildew, phytophthora root rot, cucumber mosaic virus (CMV), potyvirus, and tobacco mosaic virus (TMV) resistance. A total of 25,350 datapoints were obtained, 8.2% of which indicated resistance genotypes. Resources having perfect resistance genotype to each disease-related molecularmarker set were 58 accessions for anthracnose, 57 for powdery mildew, 246 for phytophthora root rot, and 765 for CMV. There was no resourcehaving a perfect resistance genotype of all three potyvirus-related molecular SNP markers including pvr1, pvr2 (123457), and pvr2 (689). Thirty-sevenaccessions for pvr1, 349 accessions for pvr2 (123457), and 23 accessions for pvr2 (689) were identified as resources with resistance genotype. No resource with the TMV-resistance genotype was found in this study. The resources with complex resistance genotypes of anthracnose and powderymildew were 55 accessions, and they were mainly Capsicum baccatum. Among these, seven accessions had a three-disease combined resistancegenotype with phytophthora root rot. Thirty-six accessions, 16 accessions, and nine accessions each had resistance genotypes of the three potyvirus-relatedmarkers. These resources also had CMV and phytophthora root rot resistance genotypes, and they were mainly Capsicum chinense. The statisticalanalysis considering the evaluated resistance genotypes, revealed that the anthracnose resistance genotype showed a tendency to be linked withpowdery mildew resistance genotype. The potyvirus-related marker pvr1 was positively correlated with Phytophthora root rot markers.

Index

서언
재료 및 방법
결과 및 고찰
적요
사사

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