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배추 유래 전신발현 프로모터의 동정

Identification of Constitutive Promoters Derived from Brassica rapa

김진선(Jin Sun Kim) , 임선형(Sun Hyung Lim) , 김영미(Young Mi Kim) , 이종열(Jong Yeol Lee) 저

pp.193~203 (11pages)

Publisher : KOREAN JOURNAL OF BREEDING SCIENCE
Volume/Number : KOREAN JOURNAL OF BREEDING SCIENCE Vol.50 NO.3 / 2018
Publisher : The Korean Breeding Society
Publication : Journal
Format : PDF

Abstract

English
Promoters are essential regulatory elements for efficiently expressing a gene of interest in a target tissue of a organism. Therefore, the identification of a suitable promoter is important in plant biotechnology. In this study, four promoters were selected and identified to be constitutively or tissue-specifically expressed in Brassica rapa bacterial artificial chromosome (BAC) clones using the results of transcriptomic analysis of Brassica rapa and open-source database information on Arabidopsis thaliana. The 2 kb region of the 5′ upstream was isolated from the Brassica rapa genomic DNA for each promoter. The four promoters were then fused to β-glucuronidase (gus) and green fluorescent protein (gfp) genes, and the recombinant transgenes were introduced into Arabidopsis. As a result of histochemical GUS staining, GFP fluorescence and RT-PCR, the gus gene was observed to be expressed constitutively in all tissues using all four promoters. A GUS activity assay using fluorescent 4-MUG revealed that the BR11 promoter showed similar activity to the CaMV35S promoter, while the BR4, BR15, and BR16 promoters showed 1.5, 4, and 18-fold higher activities than the CaMV35S promoter, respectively. These results indicate that these four promoters could be used to incorporate useful genes with enhanced function into crops of interest.

Index

Abstract
서언
재료 및 방법
결과 및 고찰
적요
보충자료
REFERENCES

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