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밀 반수체 계통에서 오메가-5 글리아딘 돌연변이의 선발 및 동정

Screening and Identification of Omega-5 Gliadin Mutants in Wheat Doubled-Haploid Lines

장유란(You Ran Jang) , 강천식(Chon Sik Kang) , 임선형(Sun Hyung Lim)이종열(Jong Yeol Lee) 저

pp.181~192 (12pages)

Publisher : KOREAN JOURNAL OF BREEDING SCIENCE
Volume/Number : KOREAN JOURNAL OF BREEDING SCIENCE Vol.50 NO.3 / 2018
Publisher : The Korean Breeding Society
Publication : Journal
Format : PDF

Abstract

English
Gliadin proteins are a major component of gluten proteins and important determinants of bread-making quality by conferring the viscosity and extensibility of dough, but also present significant health problems for consumers with wheat-related diseases like celiac disease or wheat allergies. In order to solve this problem, we conducted RP-HPLC analysis to profile gliadin fractions for screening the mutants deficient in gliadins from 122 wheat doubled-haploid (DH) lines cultivated by the National Institute of Crop Science. Comparing the RP-HPLC chromatogram of 122 DH lines with those of the respective parents, we found that some peaks of omega-5 gliadin were not present in 28 DH lines. Further analysis using SDS-PAGE and A-PAGE showed that the omega-5 gliadin in the parental varieties had two to three bands, but only one band in the absent 28 DH lines. The relative expression levels of all gliadin groups in the parental and mutant lines were also examined by RP-HPLC. Our study contributes to establishing a method for the rapid screening and identification of mutants missing gliadins as major epitopes of wheat-related disease in many wheat genetic resources and breeding lines as valuable information to other researchers.

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Abstract
서언
재료 및 방법
결과 및 고찰
적요
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