교보문고 SCHOLAR 스콜라
이 자료는 국가지식 연계기관과의 협약에 따라 무료로 제공됩니다.
원문을 이용하시기 위해서는 연계기관의 정책을 따르고 있으니
궁금하신 사항은 연계기관을 통해 문의하시기 바랍니다.
곧 연계기관으로 이동합니다
본문내용 바로가기
홈 > 간행물 권호
  • 발행기관: (사)한국식물생명공학회
  • 간행물:
  • 권/호:

Journal of Plant Biotechnology

  • 발행기관 : (사)한국식물생명공학회(Korean Society for Plant Biotechnology)
  • 출처구분 : 학회
  • 간행물유형 : 학술저널
  • 발행주기 : 계간 (발행월:3,6,9,12)
  • Print ISSN : 1229-2818
  • Online ISSN : 2384-1397
  • 등재정보 : KCI 등재, SCOPUS, SCI
이미지가 준비 중 입니다.
검색결과 =
7
이미지가 준비 중 입니다.
ITS 영역의 HRM 분석을 통한 참당귀(Angelica gigas Nakai)의 특이적 SNP 분자표지 개발
이신우(Shin-Woo Lee);이수진(Soo Jin Lee);한은희(Eun-Hee Han);신용욱(Yong-Wook Shin);김윤희(Yun-Hee Kim)
(사)한국식물생명공학회 / Journal of Plant Biotechnology 48권 2호 / 2021 / 71-76 (6 pages)
농수해양>기타농수해양 / KDC : 기술과학 > 농업, 농학 / KCI : 농수해양 > 농학
초록보기
Angelica is a perennial plant used widely for medicinal purposes. Information on the genetic diversity of Angelica populations is important for their conservation and germplasm utilization. Although Angelica is an important medicinal plant genus registered in South Korea, no molecular markers are currently available to distinguish individual species from other similar species in different countries, in particular, China and Japan. In this study, we developed single nucleotide polymorphism (SNP) markers derived from internal transcribed spacer regions of the nuclear ribosomal DNA to identify a distinct domestic species, Angelica gigas Nakai, via a high-resolution melting (HRM) curve analyses. We also performed HRM curve analysis of intentionally mixed genomic DNA samples from five Angelica species. Finally, we investigated A. gigas Nakai and A. sinensis using varying ratios of mixed genomic DNA templates. The SNP markers developed in this study are useful for rapidly identifying A. gigas species from different countries.
이미지가 준비 중 입니다.
배양액과 인공광 처리가 수경재배 보리의 성장과 이화학적 특성에 미치는 영향
김주성(Ju-Sung Kim)
(사)한국식물생명공학회 / Journal of Plant Biotechnology 48권 2호 / 2021 / 77-85 (9 pages)
농수해양>기타농수해양 / KDC : 기술과학 > 농업, 농학 / KCI : 농수해양 > 농학
초록보기
Hydroponic cultivation, in which crops are grown without soil and are unaffected by the weather, has many advantages over conventional soil cultivation. The crop’s growth can be further accelerated by using nutrient solution in place of water. This study investigated the growth and physicochemical properties of hydroponic barley sprouts under various nutrient solution and artificial light treatments. The shoot, root, and total plant length increased over time, with the fastest growth occurring in the nutrient solution and light-emitting diode (LED) treatments. Fresh and dry plant weights were higher in the fluorescent lamp treatment than in the LED treatment. Barley sprout powder color differed slightly by treatment, with the Hunters L value ranging from 50.79 to 53.77; Hunters a value from -6.70 to -4.42; and Hunters b value from 13.35 to 14.76. The Hunters L and Hunters b values were highest in the LED treatment, whereas the Hunters a value was relatively highest in the fluorescent lamp treatment. The total phenol content was higher in the control than in the nutrient solution treatment; however, the total flavonoid content showed the opposite pattern to that of total phenol content, being highest in plants that were grown in nutrient solution. The Trolox equivalent antioxidant capacity (TEAC) was higher in the control group than in the nutrient solution group. The ferric ion reducing antioxidant power (FRAP) was higher in the fluorescent treatment group than in the LED treatment group. The total amino acid composition ranged from 106.82 to 122.63 mg/g dry powder, with the essential amino acid composition ranging from 47.01 to 56.19 mg/g, and non-essential amino acid composition from 67.86 to 77.66 mg/g. The most frequently detected compositional amino acid was aspartic acid, followed by glutamic acid, alanine, leucine, and valine.
이미지가 준비 중 입니다.
Initiation, proliferation, and improvement of a micropropagation system for mass clonal production of banana through shoot-tip culture
Allah Jurio Khaskheli;Muharam Ali;Syad Zakir Hussain Shah;Zohra Fatima Memon;Saleem Awan;Muhammad Ibrahim Khaskheli;Mohsin Ali Khaskheli;Bilqees Magsi;Zareen Qambrani;Asad Ali Khaskheli
(사)한국식물생명공학회 / Journal of Plant Biotechnology 48권 2호 / 2021 / 86-92 (7 pages)
농수해양>기타농수해양 / KDC : 기술과학 > 농업, 농학 / KCI : 농수해양 > 농학
초록보기
The present study aimed to evaluate the initiation, proliferation potential, and mass clonal production ability of a micropropagation system for banana through tissue culture. A total of 60 explants were cultured on basal media supplemented with various concentrations of BAP and NAA. Banana plants regenerated on MS basal medium (control) without the addition of BAP + NAA showed a significantly (P < 0.05) lower survival rate with no signs of shoots up to the end of the experimental period. The results further revealed that the performance in MSS-XI medium was almost 89%, followed by MSS-IX and MSS-X media, both of which showed performance up to 88%. In contrast, the performance in the MSS-XVI medium was less than 60%, at the less duration of time and highly shoot induction detected at MSS-XIII medium. The maximum number of shoots (4.9) was observed in the medium supplemented with growth adjuster MSS-XI, followed by the MSS-XII medium (4.5). Surprisingly, the best performance was observed for the MSR-VII medium approximately 16 days after initiation, while the lowest performance was observed with MSR-XI (approximately 31 days). The maximum rooting percentage (98%) was observed in the MSR-V to MSR-VIII media (98%), while the minimum rooting percentage was observed in MSR-XI (approximately 45%)
이미지가 준비 중 입니다.
Establishment of protocol for genetic transformation of carnation with 1-aminocyclopropane-carboxylate deaminase (acdS) gene
정희영(Hui Yeong Jeong);Aung Htay Naing;김창길(・Chang Kil Kim)
(사)한국식물생명공학회 / Journal of Plant Biotechnology 48권 2호 / 2021 / 93-99 (7 pages)
농수해양>기타농수해양 / KDC : 기술과학 > 농업, 농학 / KCI : 농수해양 > 농학
초록보기
This study was conducted to develop an Agrobacterium-mediated genetic transformation protocol for the carnation cv. “Jinju” to counteract its ethylene sensitivity. The new protocol involves the use of an improved shoot regeneration medium, optimized minimal concentrations of the selective agent, a pre-culture period, and co-cultivation periods. Silver nanoparticles (NAg) added at a concentration of 2.0 µM to the Murashige and Skoog (MS) basal shoot regeneration medium supplemented with 0.1 mg/L indole-3- butyric-acid (IBA) and 0.2 mg/L thidiazuron (TDZ) improved the shoot regeneration efficiency, number of shoots per explant, and plant growth compared to the control without the addition of NAg. The phosphinothricin (PPT) concentration of 1.0 mg/L was determined to be the minimal and optimal concentration for the selection of putative transgenic plants. When the explants were infected with Agrobacterium cells harboring the acdS gene, the explants that were pre-cultured for three days induced more putative transgenic plants than those that were co-cultivated for four days. Therefore, we expect that the results of this study will benefit researchers who are developing genetic transformations of carnations.
이미지가 준비 중 입니다.
Overproduction of anthocyanin in ginseng hairy roots enhances their antioxidant, antimicrobial, and anti-elastase activities
진소라(Sora Jin);방성훈(Seounggun Bang);안민아(Min-A Ahn);이규빈(Kyubin Lee);김경환(Kyunghwan Kim);현태경(Tae Kyung Hyun)
(사)한국식물생명공학회 / Journal of Plant Biotechnology 48권 2호 / 2021 / 100-105 (6 pages)
농수해양>기타농수해양 / KDC : 기술과학 > 농업, 농학 / KCI : 농수해양 > 농학
초록보기
Genetic engineering is a potential approach to improve secondary metabolism in plants. In order to elucidate the effect of production of anthocyanin pigment 1 (PAP1) overexpression on the bioactivity of ginseng, we analyzed its antioxidant, antimicrobial, and anti-elastase activities in this study. Our results showed that PAP1 overexpression increased the production of polyphenolic compounds including anthocyanins. The antioxidant, antimicrobial, and anti-elastase activities were stronger in anthocyanin-overproducing ginseng hairy roots (AOX) than in wild ginseng hairy roots. Using a different solvent system (0, 30, 70, and 100% (v/v) EtOH), we revealed that variations in the contents of the polyphenolic compounds were highly correlated with changes in the antioxidant and antimicrobial activities of AOX. The antioxidant, antimicrobial, and anti-elastase effects of AOX highlight genetic engineering as a powerful approach to enhance the therapeutic properties of plants. Our results show that AOX could potentially have various functional applications in the cosmetic and pharmaceutical industries
이미지가 준비 중 입니다.
짝자래나무[Rhamnus yoshinoi] 가지 추출물에 의한 전립선암세포의 Wnt/β-catenin 분해 유도 활성 및 GC/MS 분석
강연경(Yeongyeong Kang);어현지(Hyun Ji Eo);김다솜(Da Som Kim);박영기(Youngki Park);박광훈(Gwang Hun Park)
(사)한국식물생명공학회 / Journal of Plant Biotechnology 48권 2호 / 2021 / 106-114 (9 pages)
농수해양>기타농수해양 / KDC : 기술과학 > 농업, 농학 / KCI : 농수해양 > 농학
초록보기
We evaluated the anti-cancer activity against human prostate cancer cells and the associated molecular mechanism of extracts from the branches of Rhamnus yoshinoi (RYB). Treatment with RYB suppressed viability of human prostate cancer cells (PC-3) and decreased protein levels of both β-catenin and T-cell factor 4 (TCF4). This was reflected in reduced TCF4 mRNA, but not decreased β- catenin mRNA. PC-3 cells were pretreated with the proteosome inhibitor MG132 before treatment with RYB, which blocked RYB-mediated down regulation of β-catenin in PC-3 cells, thus confirming that RYB promotes the proteasomal degradation of β-catenin. RYB induced β-catenin phosphorylation, and GSK-3β inhibition by LiCl blocked the phosphorylation and proteasomal degradation of β- catenin by RYB. These results suggest that GSK-3β may be an important upstream kinase for RYB-mediated regulation of β-catenin. Finally, GC/MS analysis of RYB identified 18 compounds. Based on these findings, RYB shows potential for development as a therapeutic agent for prostate cancer
이미지가 준비 중 입니다.
Effects of commercial soils on germination, early growth, and chlorophyll content of Aspilia africana, a medicinal plant
Denis Okello;Richard Komakech;김용구(Yong-Goo Kim);Endang Rahmat;정유성(Yuseong Chung);Francis Omujal;강영민(Youngmin Kang)
(사)한국식물생명공학회 / Journal of Plant Biotechnology 48권 2호 / 2021 / 115-122 (8 pages)
농수해양>기타농수해양 / KDC : 기술과학 > 농업, 농학 / KCI : 농수해양 > 농학
초록보기
Aspilia africana (Pers) C.D.Adams, a plant used for centuries in many African countries to treat diseases such as osteoporosis, malaria, tuberculosis, and diabetes, is of great pharmaceutical interest, yet there is limited scientific literature on its germination and growth. This research paper describes the effects of different commercial soils on the germination, growth, and chlorophyll content of A. africana. The germination parameters assessed included final germination percentage (FGP), mean germination time (MGT), and germination index (GI). Shoot length, leaf number, and fresh and dry weights were some of the parameters used to assess A. africana growth. The FGP was low and did not vary significantly; the MGT was 7 ~ 10 days; and the GI was significantly higher in PPS soil at 4.61 ± 0.332 days. Aspilia africana plants in HS:PPS soil showed the best overall growth, producing the highest mean leaf number (18.00 ± 1.129), longest mean shoot length (202.43 ± 13.451 mm), and highest mean fresh and dry weights (7.08 ± 1.061 g and 0.629 ± 0.112 g, respectively). The highest chlorophyll content in leaves of A. africana under HS:PPS conditions suggested a higher photosynthetic potential of plants in this soil. The best growth performance of A. africana in the HS:PPS soil could be attributed to a higher amount of certain mineral nutrients such as nitrogen, potassium, and phosphorus in the HS:PPS soil compared to the other soil categories. It is unclear why the FGP of A. africana was low and we recommend an exclusive study to investigate this further.