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홈 > 간행물 권호
  • 발행기관: 한국예방수의학회
  • 간행물:
  • 권/호:

Korean Journal of Veterinary Public Health(Korean Journal of Veterinary Public Health)

  • 발행기관 : 한국예방수의학회(The Korean Society of Preventive Veterinary Medicine)
  • 출처구분 : 학회
  • 간행물유형 : 학술저널
  • 발행주기 : 계간 (발행월:3,6,9,12)
  • pISSN : 1225-1739
  • 등재정보 : KCI 등재
Korean Journal of Veterinary Public Health
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Pathological study of Korean native pregnant cows and fetuses experimentally vaccinated with Brucella abortus RB51 in heifers and challenged with Brucella abortus 2308 in pregnancy
Pathological study of Korean native pregnant cows and fetuses experimentally vaccinated with Brucella abortus RB51 in heifers and challenged with Brucella abortus 2308 in pregnancy
You-Chan Bae;Gye-Hyeong Woo;Ha-Young Kim;Moon Her;Dong-Hee Cho;Suk-Chan Jung;Jae-Ku Oem;Jae-Won Byun;Yi-Seok Joo;O-Soo Lee;Myoung-Heon Lee
한국예방수의학회 / Korean Journal of Veterinary Public Health Vol.36, No.3 / 2012 / 109-113 (5 pages)
의약학>면역학 / KDC : 기술과학 > 의학 / KCI : 의약학 > 예방의학
초록보기
Bovine brucellosis causes abortion and infertility. The authors conducted this study in order to determine pathological lesions of Korean native cows and fetuses who received experimental vaccination with Brucella abortus RB51 and were challenged with Brucella abortus 2308. Gross and histopathological lesions in endometrium and placenta were observed in cows of the vaccinated group. Twenty-five percent of pregnant cattle in the vaccinated group showed endometritis and placentitis, which was three times lower, compared with the non-vaccinated group. The pathological lesions in the uterus and placenta in both groups were consistent with previous reports. Therefore, vaccination in heifers using Brucella abortus RB51 may not provide adequate protection against infection with Brucella abortus virulent strain.
Development of stabilized somatic cell standard solutions for calibration of electronic instruments analyzing bovine raw milk
Development of stabilized somatic cell standard solutions for calibration of electronic instruments analyzing bovine raw milk
Jin San Moon;Keum-Chan Jang;Suk-Chan Jung;Yi-Seok Joo;Ji-Ho Kim;Sung-Hwan Wee;Hye Cheong Koo;Yong Ho Park
한국예방수의학회 / Korean Journal of Veterinary Public Health Vol.36, No.3 / 2012 / 114-120 (7 pages)
의약학>면역학 / KDC : 기술과학 > 의학 / KCI : 의약학 > 예방의학
초록보기
The current standard solutions for somatic cells used for calibration of electronic somatic cell counts as reference material in raw milk are preserved with bronopol, boric acid, sodium azide, or potassium dichromate, and have a shelf-life of only up to 6 days at 4 ± 2℃. In the present study, a set of somatic cell standard solutions (SCSS) with a stability of 5 months for calibration of electronic instruments was developed. Somatic cells collected from cow’s milk and stored in a bulk tank at a dairy plant were treated with 10% formaldehyde in order to improve stability, and then separated by centrifugation. The resulting somatic cell suspension was preserved with glycerin, thimerosal, and dimethyl sulfoxide, and diluted in 3% processed skim milk solution ranging from 200,000~250,000 (low level), 350,000~450,000 (medium level), and 550,000~650,000 (high level) cells/㎖. Each SCSS was verified by direct microscope somatic cell counting (DMSCC), C-reader, and commercial standard samples. The average somatic cell count determined by DMSCC was 248, 214, 226 × 10 3 cells/㎖, 436, 382, 420 × 10 3 cells/㎖, and 612, 595, 609 × 10 3 cells/㎖. The coefficient of variation representing the repeatability of DMSCC decreased as the number of cells increased, and was <10.0% in almost all SCSS samples (range 4.6~7.1%). No statistically significant difference in somatic cell concentration was observed after storage at refrigeration temperature (2~6℃) over a period of 22 weeks (5 months). The stabilized SCSS may be useful as a reference material for determination of somatic cell count and quality control in testing of bovine raw milk.
Determination of aminoglycosides in milk by liquid chromatography with tandem mass spectrometry
Determination of aminoglycosides in milk by liquid chromatography with tandem mass spectrometry
Chae-Mi Lim;Byung-Hoon Cho;Gap-Su Chung;Seong-Wan Son
한국예방수의학회 / Korean Journal of Veterinary Public Health Vol.36, No.3 / 2012 / 121-130 (10 pages)
의약학>면역학 / KDC : 기술과학 > 의학 / KCI : 의약학 > 예방의학
초록보기
A confirmatory method based on liquid chromatography with tandem mass spectrometry was developed for deter-mination of 12 aminoglycosides in milk. Extraction of aminoglycosides from milk was performed using by liquid extrac-tion using a 10 mM phosphate buffer containing 2% (w/v) trichloroacetic acid, followed by performance of a solid-phase clean-up procedure on a hydrophilic-lipophilic balance solid-phase extraction (HLB SPE). Ion-pair chromatography, using a mixture of 20 mM heptafluorobutyric acid (HFBA) in water and acetonitrile as the mobile phase, was used for retention of aminoglycosides on a reversed-phase C 18 column. Mass spectral acquisition was performed in the multiple reaction monitoring mode, selecting two precursor ion>product ion transitions for each target compound. Satisfied recoveries (70.1~109.6%) of all aminoglycosides were demonstrated in spiked milk at three levels from 50 ng/g to 200 ng/g. The coefficients of variation ranged from 3.2% to 14.0%. The limits of quantitation (LOQs) for aminoglycosides ranged from 2.5 ng/g to 40.3 ng/g.
Determination of anthelmintic drug residues in milk using liquid chromatography with tandem mass spectrometry
Determination of anthelmintic drug residues in milk using liquid chromatography with tandem mass spectrometry
Myung-Sin Lim;Myeong-Ae Kim;Chae-Mi Lim;Byung-Hoon Cho;Jeong-Woo Kang;Seong-Wan Son
한국예방수의학회 / Korean Journal of Veterinary Public Health Vol.36, No.3 / 2012 / 131-142 (12 pages)
의약학>면역학 / KDC : 기술과학 > 의학 / KCI : 의약학 > 예방의학
초록보기
A liquid chromatographic-tandem mass spectrometric (LC-MS/MS) multi-residue method for simultaneous quantifica-tion and identification of 37 anthelmintic veterinary drug residues (including benzimidazoles, macrocyclic lactones, and flukicides, levamisole, pyrantel and niclosamide) in milk has been developed and validated. For sample preparation, we used a simple modification of the QuEChERS (quick, easy, cheap, effective, rugged and safe) method, which was ini-tially developed for analysis of pesticide residues. Anthelmintic residues were extracted into acetonitrile:methanol (9:1, v/v) using sodium chloride to induce liquid-liquid partitioning followed by dispersive solid phase extraction for cleanup. The extract was concentrated into dimethyl sulphoxide, which was used as a keeper to ensure that analytes remain in solution. Using rapid polarity switching in electrospray ionization, a single injection was capable of detecting both posi-tively and negatively charged ions within a 15 min run time. The Limit of detection (LOD) and the Limit of quan-tification (LOQ) method ranged from 0.1 ng/g to 4.4 ng/g and from 0.3 ng/g to 14.6 ng/g, respectively. Validation of the developed method was based on international guidelines. Average recoveries ranged from 70% to 120%, except for 54.7% at 0.5× MRL (rafoxanide) and 69.0% at 0.5× MRL (closantel). The coefficient of variation for the described method was less than 15% over the range of concentrations studied. The result of the method was verified successfully by participation in a proficiency study for analysis of anthelmintic drugs.
Development of real time RT-PCR method for the detection of canine influenza virus subtype H3N2
Development of real time RT-PCR method for the detection of canine influenza virus subtype H3N2
Keun Sok Na;Youn Kyoung Oh;Jin Sik Oh;Byoung Su Yoon
한국예방수의학회 / Korean Journal of Veterinary Public Health Vol.36, No.3 / 2012 / 143-150 (8 pages)
의약학>면역학 / KDC : 기술과학 > 의학 / KCI : 의약학 > 예방의학
초록보기
In the past four years, outbreaks of acute respiratory diseases associated with canine influenza H3N2 viruses in dogs and cats have been reported in South Korea and China. For prevention of disease from spread of the disease and for administration of timely medical treatments, including countermeasures for quarantine, use of a rapid and highly sen-sitive detection method are important to detection of the causative viruses. This study was conducted in order to develop a real time RT-PCR for the H3N2 subtype. It was based on primers targeting the highly homologous sequences of matrix, hemagglutinin, and neuraminidase genes. The detection limit of real time RT-PCR was 10 copies/ul with matrix and hemagglutinin genes, and 1 copy with neuraminidase genes, respectively. This real time RT-PCR was as sensitive as virus isolation in 52 clinical samples. The detection system developed in this study might provide more rapid and highly sensitive results than commercial rapid kits based on immunochromatographic assay.
Case report of laryngeal rhabdomyosarcoma in Dachshund
Case report of laryngeal rhabdomyosarcoma in Dachshund
Kyung-Hyun Lee;Soon-Seek Yoon;Woo-Hee Park;Moon-Young Rhyoo;Young-Hwa Jean;Ji-Youl Jung;You-Chan Bae;Sung-Min Park;Myung-Hyun Lee
한국예방수의학회 / Korean Journal of Veterinary Public Health Vol.36, No.3 / 2012 / 151-154 (4 pages)
의약학>면역학 / KDC : 기술과학 > 의학 / KCI : 의약학 > 예방의학
초록보기
A tissue sample of a laryngopharyngeal mass from a 9-year-old male Dachshund dog was submitted to the Animal, Plant and Fisheries Quarantine and Inspection Agency for histopathologic evaluation. The mass measured was 3.1×3.0× 5.0 ㎝ in diameter and was first detected by computed tomography. Histopathologically, the nodules consisted of large round and polygonal cells with abundant eosinophilic cytoplasm. The mitotic index was 1~3 per view under a high power field (400×). Immunohistochemically, tumor cells were positive for vimentin and desmin, but negative for cyto-keratin, S-100, and α-Smooth Muscle Actin (α-SMA). Based on these findings, the tumor was confirmed as Laryngo-pharyngeal Rhabdomyosarcoma. To the best of our knowledge, this is the first case report in Korea.
Lexicon of epidemiological models in the management of animal disease
Lexicon of epidemiological models in the management of animal disease
Hachung Yoon;Oun-Kyong Moon;Woo-Seok Jeong;Ji-Eune Lee;Youn-Ju Kim;Woo-Jin Jeon;Do-Soon Kim
한국예방수의학회 / Korean Journal of Veterinary Public Health Vol.36, No.3 / 2012 / 155-167 (13 pages)
의약학>면역학 / KDC : 기술과학 > 의학 / KCI : 의약학 > 예방의학
초록보기
Over the past decade, the magnitude and variety of modelling work in the realm of veterinary services has shown a significant increase. Accordingly, the need for enhancement of communication and cooperation among modellers (those who develop models), users (those who run models), policy makers (those who use model outputs in decision making on control policy) and coordinators (those who connect modellers with policy makers) has also shown a notable increase. In this paper, terms used in epidemiological models are listed alphabetically and explained. It is expected that development of this lexicon will help to boost communication and collaboration among those concerned with modelling work and its utilization.
Veterinary epidemiology: Concept and mission
Veterinary epidemiology: Concept and mission
Hachung Yoon;Oun-Kyong Moon;Youn-Ju Kim;Do-Soon Kim
한국예방수의학회 / Korean Journal of Veterinary Public Health Vol.36, No.3 / 2012 / 168-173 (6 pages)
의약학>면역학 / KDC : 기술과학 > 의학 / KCI : 의약학 > 예방의학
초록보기
The Veterinary Epidemiology Division of The Animal, Plant, and Fisheries Quarantine and Inspection Agency (QIA) was established in 2002. It is the first governmental organization in the Republic of Korea to be charged with the epidemiological task of managing veterinary public health. In commemorating the previous efforts of this organization, this paper describes the brief history, concept, tools, and approaches of veterinary epidemiology. The mission of veterinary epidemiology, as a leader of One Health , to improve the public health status of human and animal is also discussed.